Why use Luminol in clinical and hospital settings? What are the advantages of using Luminol? Has the highest sensitivity for detection of occult blood from the options available in the market. The Luminol is five times more sensitive than phenolphthalein, and six times more sensitive than the Malachite Green and Benzidine; Easy to use; Low cost product; Technologies that use ATP detection, are not capable of indicating the presence of red blood cells as a function of these cells were devoid of ATP; Eradication presence of hepatitis C virus and other pathogenic microorganisms in the hospital environment.
Sensitivity Benchmark. Where Using Luminol in clinical and hospital settings? The Luminol reagent applied to forensics detects: Detection of occult blood at crime scenes against life. Detection of occult blood without the use conditions of total darkness using a portable luminometer. Detection of cells in firearms by luminometer. Where Using Luminol in fridges: Hand cutting instruments; Cutting equipment and storage; Packaging; Uniforms and personal protective equipment; Effluent from the refrigerator, etc.
What are the advantages of using Luminol in Fridges to certify the Quality? A method of determining the presence of blood in and on a dental needle after the administration of local anesthetic. The detection of blood on dental surgery surfaces and equipment following dental hygiene treatment.
Increased luminol enhanced chemiluminescence from peripheral granulocytes in juvenile periodontitis. Scand J Dent Res. Endodontics deals with diseases of the dental pulp and periapical tissues, which are highly vascularized. During root canal treatment, endodontic files may come into contact with blood from these tissues and become carrier of viruses, such as that of hepatitis 16 16 Ramadan AA.
Removing hepatitis C virus from polytetrafluoroethylene-coated orthodontic archwires and other dental instruments. East Mediterr Health J. Therefore, the aim of this study was to investigate the efficacy of luminol in detecting blood in endodontic files before and after the sterilization process. This study has evaluated endodontic files used during endodontic treatment performed by final-year undergraduate students. All patients signed an informed consent form agreeing in participating of the research.
The 5-amino-2,3-dihydro-1,4-ftalazinadione used in this study was obtained according to a synthetic method developed by Lopes et al. The use of chemiluminescence of luminol in forensic medicine and toxicology. Identification of blood stains. Dtsch Z Gesamte Gerichtl Med. Fifty endodontic files used during instrumentation of the apical third of root canals were included in this study.
Next, the files were individually stored in sterile glass vials with screw cap. Two examiners analyzed the endodontic files and confirmed the presence or absence of blood stains visible to naked eye. In a dark room, the file was covered with both solutions luminol and hydrogen peroxide in order to detect any trace of blood.
If positive, the surface of the instrument becomes pigmented with fluorescent blue light, which is noticeable in an environment in the absence of light. If negative, no change occurs in the surface under these conditions. The files were then washed and submitted to disinfection procedures and sterilization by autoclave according to bio-safety standards. Next, the endodontic files to be used in future clinical interventions were retested with the aid of luminol.
In this phase, the endodontic files were collected from the autoclaved vials and again submitted to luminol test under the same light conditions previously described. It was expected that the compound would not detect blood in the dental instruments after the sterilization process. The person who handled the contaminated material during the tests wore protective equipment, including gloves, mask, cap, goggles and disposable laboratory coat, to prevent contamination with microorganisms from blood, saliva, body fluids, secretions and excretions possibly present in the dental instruments analyzed.
The same amount of blood 2. This material was stored under room temperature in a closed container, with the necessary information for handling by authorized persons only.
Half of the slides prepared underwent successive luminol tests, whereas the other half was submitted to autoclave sterilization. The slides were stored and analyzed after 24 hours. Zero 0 : unable to identify blood tissue by naked eye or in the presence of luminol, before and after the sterilization process.
One 1 : slight presence of blood tissue was identified by naked eyes or in the presence of luminol, before and after the sterilization process. Media Center. Contact DPS. It looks like your browser does not have JavaScript enabled. Please turn on JavaScript and try again. Forensic Science Services. Expand All Collapse All. Page Content. Spanish Hmong Somali. If there are any blood traces in the area, they will glow.
Sign up for our Newsletter! Mobile Newsletter banner close. Mobile Newsletter chat close. Mobile Newsletter chat dots. Mobile Newsletter chat avatar.
0コメント